“Comparative Evaluation Of Biofilm Development Of Candida Albicans On Abraded Surfaces Of Heat Cure PMMA And Flexi Denture Material”:An In Vitro Study

Purpose: Study was to compare the biofilm development of candida albicans on two different types of abraded surfaces of heat cure PMMA and flexi denture material. Materials And Methods: 4 blocks of each heat cure resin and flexible denture material were prepared of 10*10*10mm and 10*10*3mm in size respectively. All blocks were then subjected to abrasion with p100 grit size dry sand paper or with denture cleansing brush. One colony of Candida albicans GDH 2346 (NCYC 1467) cultured on Sabouraud dextrose agar (SBA) for 48 hrs and was used to inoculate 10 mL of Peptone water and sugar broth with samples at 37°C for 24hrs. Followed by which gram staining was performed and semiquantification was done after inoculating on SBA for 18 hours at 37oC. Results: The highest mean colony forming unit was recorded in heat cure PMMA, and lowest mean colony forming unit was recorded in flexible denture material. Conclusion: Flexible denture material has lesser biofilm development and candidal count as compared to heat cure acrylic resin.